//genesis // kkit Version 2 dumpfile // Saved on Thu Jun 27 11:31:08 1996 include kkit FASTDT = 0.005 SIMDT = 0.005 CONTROLDT = 1 PLOTDT = 1 MAXTIME = 2000 kparms //genesis initdump -version 3 -ignoreorphans 1 simobjdump table input output alloced step_mode stepsize simobjdump xtree path script namemode sizescale simobjdump xcoredraw xmin xmax ymin ymax simobjdump xtext editable simobjdump xgraph xmin xmax ymin ymax overlay simobjdump xplot pixflags flags script fg ysquish do_slope wy simobjdump kpool CoTotal CoInit Co CoRemaining n nInit nTotal nMin vol \ slave_enable keepconc notes xtree_fg_req xtree_textfg_req simobjdump xtab input output alloced step_mode stepsize notes editfunc \ xtree_fg_req xtree_textfg_req plotfield manageclass baselevel last_x last_y \ is_running simobjdump group notes editfunc xtree_fg_req xtree_textfg_req plotfield \ manageclass expanded movealone simobjdump kreac A B kf kb is_active notes editfunc xtree_fg_req \ xtree_textfg_req plotfield manageclass simobjdump kenz CoComplexInit CoComplex nComplexInit nComplex vol sA pA eA B \ k1 k2 k3 keepconc usecomplex ksum notes editfunc xtree_fg_req \ xtree_textfg_req plotfield manageclass link_to_manage simundump group /kinetics/PKC 0 "" edit_group blue black x group 0 0 simundump kpool /kinetics/PKC/PKC-Ca 0 0.28282 3.7208e-17 0.11562 0.1672 \ 69374 2.2325e-11 1.6969e+05 0 6e+05 0 1 "" red black simundump kreac /kinetics/PKC/PKC-act-by-Ca 0 34687 34687 1e-06 0.5 1 \ "Need est of rate of assoc of Ca and PKC. Assume it is fast\nThe original parameter-searched kf of 439.4 has been\nscaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085\nRaised kf to 1e-6 to match Ca curve, kb to .5\n" \ edit_reac white blue A group simundump kreac /kinetics/PKC/PKC-act-by-DAG 0 83248 83248 1.3333e-08 8.6348 \ 1 \ "Need est of rate. Assume it is fast\nObtained from param search\nkf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991.\nkf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of\nShinomura data.\n26 June 1996: Corrected DAG data: reduce kf 15x from \n2e-7 to 1.333e-8" \ edit_reac white blue A group simundump kreac /kinetics/PKC/PKC-Ca-to-memb 0 88137 88137 1.2705 3.5026 1 "" \ edit_reac white blue A group simundump kreac /kinetics/PKC/PKC-DAG-to-memb 0 9641 9641 1 0.1 1 \ "Raise kb from .087 to 0.1 to match data from Shinomura et al.\nLower kf from 1.155 to 1.0 to match data from Shinomura et al." \ edit_reac white blue A group simundump kreac /kinetics/PKC/PKC-act-by-Ca-AA 0 4162.4 4162.4 2e-09 0.1 1 \ "Schaechter and Benowitz We have to increase Kf for conc scaling\nChanged kf to 2e-9 on Sept 19, 94. This gives better match.\n" \ edit_reac white blue A group simundump kreac /kinetics/PKC/PKC-act-by-DAG-AA 0 46827 46827 2 0.2 1 \ "Assume slowish too. Schaechter and Benowitz" edit_reac white blue A group simundump kpool /kinetics/PKC/PKC-DAG-AA* 0 0.12061 4.9137e-18 0.39023 \ -0.26962 2.3414e+05 2.9482e-12 72366 0 6e+05 0 1 "" cyan blue simundump kpool /kinetics/PKC/PKC-Ca-AA* 0 0.16961 1.75e-16 0.069374 0.10024 \ 41624 1.05e-10 1.0177e+05 0 6e+05 0 1 "" orange blue simundump kpool /kinetics/PKC/PKC-Ca-memb* 0 0.10258 1.3896e-17 0.041939 \ 0.060644 25163 8.3376e-12 61549 0 6e+05 0 1 "" pink blue simundump kpool /kinetics/PKC/PKC-DAG-memb* 0 0.023754 9.4352e-21 0.16068 \ -0.13693 96410 5.6611e-15 14252 0 6e+05 0 1 "" yellow blue simundump kpool /kinetics/PKC/PKC-basal* 0 0.0432 0.02 0.0019271 0.041273 \ 1156.2 12000 25920 0 6e+05 0 1 "" pink blue simundump kreac /kinetics/PKC/PKC-basal-act 0 57812 57812 1 50 1 \ "Initial basal levels were set by kf = 1, kb = 20. In model, though, the\nbasal levels of PKC activity are higher." \ edit_reac white blue A group simundump kpool /kinetics/PKC/PKC-AA* 0 1 1.8133e-17 0.0057812 0.99422 3468.7 \ 1.088e-11 6e+05 0 6e+05 0 1 "" cyan blue simundump kreac /kinetics/PKC/PKC-act-by-AA 0 346.87 346.87 2e-10 0.1 1 \ "Raise kf from 1.667e-10 to 2e-10 to get better match to data." edit_reac \ white blue A group simundump kpool /kinetics/PKC/PKC-Ca-DAG 0 0.0017993 8.4632e-23 0.016068 \ -0.014269 9641 5.0779e-17 1079.6 0 6e+05 0 1 "" white blue simundump kreac /kinetics/PKC/PKC-n-DAG 0 5203 5203 1e-09 0.1 1 \ "kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991\ncloser analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8).\nFurther tweak. To get sufficient AA synergy, increase kf to 1.5e-8\n26 June 1996: Corrected DAG levels: reduce kf by 15x from\n1.5e-8 to 1e-9" \ edit_reac white blue A group simundump kpool /kinetics/PKC/PKC-DAG 0 0.08533 1.161e-16 0.086717 -0.0013868 \ 52030 6.9661e-11 51198 0 6e+05 0 1 "CoInit was .0624\n" white blue simundump kreac /kinetics/PKC/PKC-n-DAG-AA 0 46827 46827 3e-08 2 1 \ "Reduced kf to 0.66X to match Shinomura et al data.\nInitial: kf = 3.3333e-9\nNew: 2e-9\nNewer: 2e-8\nkb was 0.2\nnow 2." \ edit_reac white blue A group simundump kpool /kinetics/PKC/PKC-DAG-AA 0 0.012092 2.5188e-19 0.039023 \ -0.02693 23414 1.5113e-13 7255.5 0 6e+05 0 1 "" white blue simundump kpool /kinetics/PKC/PKC-cytosolic 0 1.5489 1 0.096353 1.4526 57812 \ 6e+05 9.2935e+05 0 6e+05 0 1 \ "Marquez et al J. Immun 149,2560(92) est 1e6/cell for chromaffin cells\nWe will use 1 uM as our initial concen\n" \ white blue simundump kpool /kinetics/DAG 1 150 150 150 -111 9e+07 9e+07 9e+07 0 6e+05 6 \ 0 \ "The conc of this has been a problem. Schaecter and Benowitz use 50 uM,\nbut Shinomura et al have < 5. So I have altered the DAG-dependent \nrates in the PKC model to reflect this." \ green black simundump kpool /kinetics/Ca 1 1 1 1 -9 6e+05 6e+05 6e+05 0 6e+05 6 0 "" red \ black simundump kpool /kinetics/AA 0 50 50 50 0 3e+07 3e+07 3e+07 0 6e+05 6 1 "" \ darkgreen black simundump kpool /kinetics/PKC-active 0 0.02 0 0.66993 5.5604e-08 4.0196e+05 \ 12000 4.0196e+05 0 6e+05 2 1 "Conc of PKC in brain is about 1 uM (?)" \ yellow black simundump kenz /kinetics/PKC-active/PKC-act-raf 1 0 5.5604e-08 0 0.033363 \ 6e+05 0.5338 0.13345 0.66725 0.66725 1.66e-06 16 4 0 0 20 \ "Rate consts from Chen et al Biochem 32, 1032 (1993)\nk3 = k2 = 4\nk1 = 9e-5\nrecalculated gives 1.666e-5, which is not very different.\nLooks like k3 is rate-limiting in this case: there is a huge amount\nof craf locked up in the enz complex. Let us assume a 10x\nhigher Km, ie, lower affinity. k1 drops by 10x.\nAlso changed k2 to 4x k3.\nLowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC" \ edit_enz red yellow CoComplex kpool enzproto_to_pool_link simundump xtab /kinetics/AA-conc-series 0 0 50 1 1 0 "" edit_xtab white red \ output group 0 100 0.009902649474 3 loadtab /kinetics/AA-conc-series table 1 100 0 400 \ 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 10 10 10 10 10 10 10 10 10 10 \ 10 10 10 10 10 10 10 10 10 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 \ 25 25 25 25 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 100 \ 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 simundump xtab /kinetics/Ca-conc-series 0 0 1 1 1 0 "" edit_xtab white red \ output group 1e-6 0 0 3 loadtab /kinetics/Ca-conc-series table 1 100 0 400 \ 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 \ 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.01 0.01 0.01 0.01 \ 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 \ 0.01 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 \ 0.1 0.1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 10 10 10 10 10 10 10 10 10 \ 10 10 10 10 10 10 10 10 10 10 10 simundump xtab /kinetics/Ca-conc-step 0 99.755 1 1 1 0.005 "" edit_xtab white \ red output group 1e-4 0 0 3 loadtab /kinetics/Ca-conc-step table 1 100 0 100 \ 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 \ 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 \ 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 1 1 1 1 1 1 1 1 1 1 1 1 \ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 simundump xtab /kinetics/DAG-conc-series 1 0 18 1 1 0 \ "A remarkably small conc range." edit_xtab white red output group 0 0 0 3 loadtab /kinetics/DAG-conc-series table 1 100 0 2000 \ 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 18 18 18 18 18 18 18 18 18 18 \ 18 18 18 18 18 18 18 18 18 37.5 37.5 37.5 37.5 37.5 37.5 37.5 37.5 37.5 37.5 \ 37.5 37.5 37.5 37.5 37.5 37.5 37.5 37.5 37.5 37.5 75 75 75 75 75 75 75 75 75 \ 75 75 75 75 75 75 75 75 75 75 75 112.5 112.5 112.5 112.5 112.5 112.5 112.5 \ 112.5 112.5 112.5 112.5 112.5 112.5 112.5 112.5 112.5 112.5 112.5 112.5 \ 112.5 simundump xgraph /graphs/conc1 0 0 2000 0 130.24 0 simundump xgraph /graphs/conc2 0 0 2000 0 0.595 0 simundump xplot /graphs/conc1/AA.Co 3 524288 3 \ "delete_plot.w ; edit_plot.D " darkgreen 0.55 0 1 simundump xplot /graphs/conc1/DAG.Co 3 524288 3 \ "delete_plot.w ; edit_plot.D " green 0.55 0 1 simundump xplot /graphs/conc2/Ca.Co 3 524288 3 \ "delete_plot.w ; edit_plot.D " red 0.005 0 1 simundump xplot /graphs/conc2/PKC-active.Co 3 524288 3 \ "delete_plot.w ; edit_plot.D " yellow 0.005 0 1 simundump xplot /graphs/conc2/PKC-DAG.Co 3 524288 3 \ "delete_plot.w ; edit_plot.D " white 0.005 0 1 simundump xgraph /moregraphs/conc3 0 0 2000 0 1 0 simundump xgraph /moregraphs/conc4 0 0 2000 0 1 0 simundump xcoredraw /edit/draw 0 -12.316 7.6873 -10.818 9.1849 simundump xtree /edit/draw/tree 0 /kinetics/##[] \ "edit_elm.D ; drag_from_edit.w " auto 0.6 call /edit/draw/tree ADDMSGARROW \ select all all all none -1000 0 \ "" "" "" call /edit/draw/tree ADDMSGARROW \ /kinetics/##[] /kinetics/##[TYPE=group] all all none -1001 0 \ "echo.p dragging onto group ; thing_to_group_add.p " "" "" call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kpool] SUMTOTAL kpool darkgreen -1002 0 \ "echo.p dragging to for sumtotal" "pool_to_pool_add_sumtot.p " "pool_to_pool_drop_sumtot.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kpool] CONSERVE kpool blue -1003 0 \ "echo.p dragging to for conserve" "pool_to_pool_add_consv.p " "pool_to_pool_drop_consv.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kreac] /kinetics/##[TYPE=kpool] REAC kpool none -1004 0 \ "echo.p dragging to for product" "reac_to_pool_add.p " "reac_to_pool_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kreac] SUBSTRATE kreac green -1005 0 \ "echo.p dragging to for substrate" "pool_to_reac_add.p " "pool_to_reac_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kreac] PRODUCT kreac green -1006 1 \ "" "" "" call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kenz] /kinetics/##[TYPE=kpool] MM_PRD kpool red -1007 0 \ "echo.p dragging to for enz product" "enz_to_pool_add.p " "enz_to_pool_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kenz] SUBSTRATE kenz red -1008 0 \ "echo.p dragging to for enz substrate" "pool_to_enz_add.p " "pool_to_enz_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kenz] /kinetics/##[TYPE=kpool] SUMTOTAL kpool darkgreen -1009 0 \ "" "" "" call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kenz] /kinetics/##[TYPE=kpool] CONSERVE kpool blue -1010 0 \ "" "" "" call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kenz] INTRAMOL kenz white -1011 0 \ "" "" "" call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=stim] /kinetics/##[TYPE=kpool] SLAVE kpool orange -1012 0 \ "echo.p dragging to for stimulus" "stim_to_pool_add.p " "stim_to_pool_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=stim] /kinetics/##[TYPE=stim] INPUT stim orange -1013 0 \ "echo.p dragging to for stim" "stim_to_stim_add.p " "stim_to_stim_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=xtab] /kinetics/##[TYPE=kpool] SLAVE kpool orange -1014 0 \ "echo.p dragging to for xtab" "xtab_to_pool_add.p " "xtab_to_pool_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=xtab] /kinetics/##[TYPE=xtab] INPUT xtab orange -1015 0 \ "echo.p dragging to for xtab" "xtab_to_xtab_add.p " "xtab_to_xtab_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=xtab] INPUT xtab hotpink -1016 0 \ "echo.p dragging to for xtab" "pool_to_xtab_add.p " "pool_to_xtab_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kchan] /kinetics/##[TYPE=kpool] REAC kpool none -1017 0 \ "echo.p dragging to for chan product" "chan_to_pool_add.p " "chan_to_pool_drop.p " call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kchan] PRODUCT kchan orange -1018 1 \ "" "" "" call /edit/draw/tree ADDMSGARROW \ /kinetics/##[TYPE=kpool] /kinetics/##[TYPE=kchan] SUBSTRATE kchan orange -1019 0 \ "echo.p dragging to for chan substrate" "pool_to_chan_add.p " "pool_to_chan_drop.p " call /edit/draw/tree UNDUMP 31 \ "/kinetics/PKC" -3.04929 8.21631 0 \ "/kinetics/PKC/PKC-Ca" -4.07517 1.51085 0 \ "/kinetics/PKC/PKC-act-by-Ca" -4.07517 -0.122947 0 \ "/kinetics/PKC/PKC-act-by-DAG" -2.06118 0.693953 0 \ "/kinetics/PKC/PKC-Ca-to-memb" -3.79738 4.25331 0 \ "/kinetics/PKC/PKC-DAG-to-memb" -2.61677 2.73621 0 \ "/kinetics/PKC/PKC-act-by-Ca-AA" -0.78797 3.81568 0 \ "/kinetics/PKC/PKC-act-by-DAG-AA" 1.24916 3.23218 0 \ "/kinetics/PKC/PKC-DAG-AA*" 0.600985 5.53701 0 \ "/kinetics/PKC/PKC-Ca-AA*" -0.602776 6.29557 0 \ "/kinetics/PKC/PKC-Ca-memb*" -2.77881 6.52897 0 \ "/kinetics/PKC/PKC-DAG-memb*" -1.82969 5.50784 0 \ "/kinetics/PKC/PKC-basal*" -4.7465 5.56619 0 \ "/kinetics/PKC/PKC-basal-act" -4.97799 3.05713 0 \ "/kinetics/PKC/PKC-AA*" 1.78159 6.82072 0 \ "/kinetics/PKC/PKC-act-by-AA" -4.99245 -1.86537 0 \ "/kinetics/PKC/PKC-Ca-DAG" 0.230597 1.8026 0 \ "/kinetics/PKC/PKC-n-DAG" -3.0103 -1.99016 0 \ "/kinetics/PKC/PKC-DAG" -0.996314 -1.08573 0 \ "/kinetics/PKC/PKC-n-DAG-AA" -1.22781 -2.95294 0 \ "/kinetics/PKC/PKC-DAG-AA" 0.624134 0.227153 0 \ "/kinetics/PKC/PKC-cytosolic" -6.13155 0.597108 0 \ "/kinetics/DAG" -3.20515 -4.71675 0 \ "/kinetics/Ca" -8.3874 -2.76345 0 \ "/kinetics/AA" -3.28976 -9.33758 0 \ "/kinetics/PKC-active" 2.13247 8.47704 0 \ "/kinetics/PKC-active/PKC-act-raf" 6.26075 9.14727 0 \ "/kinetics/AA-conc-series" -1.20937 -11.7853 0 \ "/kinetics/Ca-conc-series" -8.86992 -6.55545 0 \ "/kinetics/Ca-conc-step" -7.27763 -4.96314 0 \ "/kinetics/DAG-conc-series" 1.83714 -5.91609 0 simundump xtext /file/notes 0 1 xtextload /file/notes \ "Corrected DAG levels. 15x scale factor" \ "applied. Going through dose-R " \ "curves to check that all is still well." addmsg /kinetics/PKC/PKC-act-by-Ca /kinetics/PKC/PKC-Ca REAC B A addmsg /kinetics/PKC/PKC-act-by-DAG /kinetics/PKC/PKC-Ca REAC A B addmsg /kinetics/PKC/PKC-Ca-to-memb /kinetics/PKC/PKC-Ca REAC A B addmsg /kinetics/PKC/PKC-act-by-Ca-AA /kinetics/PKC/PKC-Ca REAC A B addmsg /kinetics/PKC/PKC-cytosolic /kinetics/PKC/PKC-act-by-Ca SUBSTRATE n addmsg /kinetics/Ca /kinetics/PKC/PKC-act-by-Ca SUBSTRATE n addmsg /kinetics/PKC/PKC-Ca /kinetics/PKC/PKC-act-by-Ca PRODUCT n addmsg /kinetics/DAG /kinetics/PKC/PKC-act-by-DAG SUBSTRATE n addmsg /kinetics/PKC/PKC-Ca /kinetics/PKC/PKC-act-by-DAG SUBSTRATE n addmsg /kinetics/PKC/PKC-Ca-DAG /kinetics/PKC/PKC-act-by-DAG PRODUCT n addmsg /kinetics/PKC/PKC-Ca /kinetics/PKC/PKC-Ca-to-memb SUBSTRATE n addmsg /kinetics/PKC/PKC-Ca-memb* /kinetics/PKC/PKC-Ca-to-memb PRODUCT n addmsg /kinetics/PKC/PKC-Ca-DAG /kinetics/PKC/PKC-DAG-to-memb SUBSTRATE n addmsg /kinetics/PKC/PKC-DAG-memb* /kinetics/PKC/PKC-DAG-to-memb PRODUCT n addmsg /kinetics/PKC/PKC-Ca /kinetics/PKC/PKC-act-by-Ca-AA SUBSTRATE n addmsg /kinetics/AA /kinetics/PKC/PKC-act-by-Ca-AA SUBSTRATE n addmsg /kinetics/PKC/PKC-Ca-AA* /kinetics/PKC/PKC-act-by-Ca-AA PRODUCT n addmsg /kinetics/PKC/PKC-DAG-AA* /kinetics/PKC/PKC-act-by-DAG-AA PRODUCT n addmsg /kinetics/PKC/PKC-DAG-AA /kinetics/PKC/PKC-act-by-DAG-AA SUBSTRATE n addmsg /kinetics/PKC/PKC-act-by-DAG-AA /kinetics/PKC/PKC-DAG-AA* REAC B A addmsg /kinetics/PKC/PKC-act-by-Ca-AA /kinetics/PKC/PKC-Ca-AA* REAC B A addmsg /kinetics/PKC/PKC-Ca-to-memb /kinetics/PKC/PKC-Ca-memb* REAC B A addmsg /kinetics/PKC/PKC-DAG-to-memb /kinetics/PKC/PKC-DAG-memb* REAC B A addmsg /kinetics/PKC/PKC-basal-act /kinetics/PKC/PKC-basal* REAC B A addmsg /kinetics/PKC/PKC-cytosolic /kinetics/PKC/PKC-basal-act SUBSTRATE n addmsg /kinetics/PKC/PKC-basal* /kinetics/PKC/PKC-basal-act PRODUCT n addmsg /kinetics/PKC/PKC-act-by-AA /kinetics/PKC/PKC-AA* REAC B A addmsg /kinetics/AA /kinetics/PKC/PKC-act-by-AA SUBSTRATE n addmsg /kinetics/PKC/PKC-AA* /kinetics/PKC/PKC-act-by-AA PRODUCT n addmsg /kinetics/PKC/PKC-cytosolic /kinetics/PKC/PKC-act-by-AA SUBSTRATE n addmsg /kinetics/PKC/PKC-act-by-DAG /kinetics/PKC/PKC-Ca-DAG REAC B A addmsg /kinetics/PKC/PKC-DAG-to-memb /kinetics/PKC/PKC-Ca-DAG REAC A B addmsg /kinetics/PKC/PKC-cytosolic /kinetics/PKC/PKC-n-DAG SUBSTRATE n addmsg /kinetics/DAG /kinetics/PKC/PKC-n-DAG SUBSTRATE n addmsg /kinetics/PKC/PKC-DAG /kinetics/PKC/PKC-n-DAG PRODUCT n addmsg /kinetics/PKC/PKC-n-DAG /kinetics/PKC/PKC-DAG REAC B A addmsg /kinetics/PKC/PKC-n-DAG-AA /kinetics/PKC/PKC-DAG REAC A B addmsg /kinetics/PKC/PKC-DAG /kinetics/PKC/PKC-n-DAG-AA SUBSTRATE n addmsg /kinetics/AA /kinetics/PKC/PKC-n-DAG-AA SUBSTRATE n addmsg /kinetics/PKC/PKC-DAG-AA /kinetics/PKC/PKC-n-DAG-AA PRODUCT n addmsg /kinetics/PKC/PKC-n-DAG-AA /kinetics/PKC/PKC-DAG-AA REAC B A addmsg /kinetics/PKC/PKC-act-by-DAG-AA /kinetics/PKC/PKC-DAG-AA REAC A B addmsg /kinetics/PKC/PKC-act-by-Ca /kinetics/PKC/PKC-cytosolic REAC A B addmsg /kinetics/PKC/PKC-basal-act /kinetics/PKC/PKC-cytosolic REAC A B addmsg /kinetics/PKC/PKC-act-by-AA /kinetics/PKC/PKC-cytosolic REAC A B addmsg /kinetics/PKC/PKC-n-DAG /kinetics/PKC/PKC-cytosolic REAC A B addmsg /kinetics/PKC/PKC-act-by-DAG /kinetics/DAG REAC A B addmsg /kinetics/PKC/PKC-n-DAG /kinetics/DAG REAC A B addmsg /kinetics/DAG-conc-series /kinetics/DAG SLAVE output addmsg /kinetics/PKC/PKC-act-by-Ca /kinetics/Ca REAC A B addmsg /kinetics/Ca-conc-series /kinetics/Ca SLAVE output addmsg /kinetics/PKC/PKC-act-by-Ca-AA /kinetics/AA REAC A B addmsg /kinetics/PKC/PKC-act-by-AA /kinetics/AA REAC A B addmsg /kinetics/PKC/PKC-n-DAG-AA /kinetics/AA REAC A B addmsg /kinetics/AA-conc-series /kinetics/AA SLAVE output addmsg /kinetics/PKC/PKC-DAG-AA* /kinetics/PKC-active SUMTOTAL n nInit addmsg /kinetics/PKC/PKC-Ca-memb* /kinetics/PKC-active SUMTOTAL n nInit addmsg /kinetics/PKC/PKC-Ca-AA* /kinetics/PKC-active SUMTOTAL n nInit addmsg /kinetics/PKC/PKC-DAG-memb* /kinetics/PKC-active SUMTOTAL n nInit addmsg /kinetics/PKC/PKC-basal* /kinetics/PKC-active SUMTOTAL n nInit addmsg /kinetics/PKC/PKC-AA* /kinetics/PKC-active SUMTOTAL n nInit addmsg /kinetics/PKC-active/PKC-act-raf /kinetics/PKC-active REAC sA B addmsg /kinetics/PKC-active/PKC-act-raf /kinetics/PKC-active CONSERVE nComplex nComplexInit addmsg /kinetics/PKC-active /kinetics/PKC-active/PKC-act-raf ENZYME n addmsg /kinetics/AA /graphs/conc1/AA.Co PLOT Co *AA.Co *darkgreen addmsg /kinetics/DAG /graphs/conc1/DAG.Co PLOT Co *DAG.Co *green addmsg /kinetics/Ca /graphs/conc2/Ca.Co PLOT Co *Ca.Co *red addmsg /kinetics/PKC-active /graphs/conc2/PKC-active.Co PLOT Co *PKC-active.Co *yellow addmsg /kinetics/PKC/PKC-DAG /graphs/conc2/PKC-DAG.Co PLOT Co *PKC-DAG.Co *white enddump // End of dump call /edit/draw/tree RESET reset